Sensitive determination of cell number using the CyQUANT cell

CyQUANT® NF Cell Proliferation Assay and a moderate but consistent protocol Briefly, 24h post miR transfection, 5000 cells were

The modified CyQuant® assay eliminates the requirement for labour intensive fluorescent labelling protocols The basic protocol is as

cyquant protocol Read the absorbance for Cyquant assay using the plate reader with excitation at 485 nm and emission at 530 nm 6 Interpolate the standard curve

cyquant protocol The assay protocol was configured to analyze 500 cells per well to measure CyQUANT® are shown Linear regression for both correlation plots